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Similar changes in the expression of voltage-gated Ca 2+ channels (VGCCs) have been reported following chronic alcohol exposure (Dolin et al ., 1987)

LCMS/MS was carried out on an UltiMate 3000 nanoLC coupled to a Q Exactive Plus Orbitrap (Thermo Fisher Scientific) using aqueous and acetonitrile mobile phases, each supplemented with 0.1% formic acid

55 This discrepancy may arise from bile dynamics: short-term alcohol consumption transiently raises acetaldehyde levels (later excreted), while chronic use prolongs gallbladder exposure, possibly increasing GBC risk