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Description

Mycoplasma gallisepticum -induced alterations in chicken red blood cells

Injections were performed under deep anesthesia using a combination of ketamine (90 mg/kg) and xylazine (10 mg/kg)

Cells were sorted using a FACS LSRFortessa (BD Biosciences) to determine the percentage of annexin V- and/or 7-AAD-positive cells using a 488 nm laser for annexin V-FITC and annexin V-PE and a 675 nm laser for7-AAD