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After washing 3 times with PBS, neurons were incubated with 5.5 M pHluorin-labelled Syt1 nanobody in the presence of either 30mM KCl or 1M TTX (Alomone labs) to stimulate neurons or to silence them, respectively, for a total of 20 min at 37 C in a humid chamber

Since large clinical trials have found daily low-dose aspirin provides little to no cardiovascular benefit in those without a prior history of a coronary event or stroke, guidelines do not recommend its use for such individuals, except in those with exceptionally high cardiovascular risk and no other factors that increase bleeding risk