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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

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Cyclooxygenase 2-selective and nonselective nonsteroidal anti-inflammatory drugs induce oxidative stress by up-regulating vascular NADPH oxidases

Assisted reproductive technologies (ART) adjuncts Use of peptides to supplement culture media or improve embryo transfer protocols or endometrial priming could improve implantation rates and embryo health

Neither of these roles has been disproved, but interest in them became somewhat overshadowed by conceptual developments in other directions