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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

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Given the quality questions and absence of clinical support, this is poor value compared to many other interventions with better evidence and lower risk profiles

Stay vigilant about hydration if symptoms develop, as dehydration poses the greatest danger

Patients notice effects on sleep depth and circadian rhythm first, which is a direct reflection of its pineal gland origins