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(44) has shown that apigenin can improve the symptoms of arthritis in CFA rats and its anti-inflammatory mechanism may be mediated by reducing the expression of cytokines (IL-1, IL-6 and TNF-) and inhibiting the P2X7/NF-B signaling pathway

After all non-retained components are washed off the column, binding solute or together with ligand as solute-ligand complex are eluted by presence of mobile phase which was prepared in suitable pH, ionic strength and solvent composition for solute-ligand binding