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Prostate cancer statistics, 2025

doi: 10.3389/fphar.2025.1529194 47

293 T cell IL-1 turn over and caspase-1 cleavage assays For IL-1 turnover assays, confluent 293T cells plated on 10 cm tissue culture plates were transfected using lipofectamine 2000 (Life Technologies) with 10 g of murine IL-1 and point mutant variants (cloned into pMIGMCS) and after 24 h cells harvested and plated onto 24-well tissue culture plates and left to settle overnight