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Abstract Purpose MTHFR, one of the major enzymes in the folate cycle, is known to acquire single-nucleotide polymorphisms that significantly reduce its activity, resulting in an increase in circulating homocysteine

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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

J., Foroozesh, M., Church, D