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doi: 10.1016/j.freeradbiomed.2015.12.004

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Neural stem cell-derived EVs, on the other hand, deliver functional mitochondria to recipient cells, thereby modulating metabolic activity [51]

After washing once with RIPA buffer, three times with 1% (v/v) SDS, 8 M Urea in PBS, once with 1% (v/v) SDS in PBS and two times with PBS, pulled down proteins were either eluted by 2x Laemmli, including 100 M biotin, for western blot analysis or on-bead digested for mass spectrometry evaluation (see mass spectrometry methods)