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Immunofluorescence Thin sections of mouse brain and eyeball were washed with PBS, permeabilized with PBS containing 0.05% (v/v) saponin and 5% (v/v) normal goat serum for 3 min, and treated with PBS containing 1.5% normal goat serum for 1 h to block nonspecific binding

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Due to its role in DNA synthesis and red blood cell formation, B12 deficiency may result in anaemia (with symptoms such as pallor, dizziness, palpitations and shortness of breath) and damaged mucous membranes

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In cases of hypothyroidism , the thyroid gland becomes underactive , which can significantly slow down all the functions directly affected by its hormones, and can be triggered by Hashimotos thyroiditis