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The diluent type selection (bacteriostatic water or sterile water) is for labelling purposes only and doesnt affect the calculation

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For 53BP1 staining, coverslips were washed with ice-cold PBS, fixed with 4% (v/v) formaldehyde in PBS for 15 min in the dark at RT, and then permeabilized with 0.1% (v/v) Triton X-100 for 10 min at RT

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AOD 9604 works by binding to the same receptors in the body that are responsible for fat metabolism