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2018) and Oxford Nanopore Technologies (ONT) (Lu et al

Metabolites were extracted using ultrasonic treatment in an ice bath for 30 min and then centrifuged for 10 min at 12,000 rpm

Following incubation, oocytes and blastocysts were washed three times in DPBS (1x) containing PVP (0.1%) and immediately imaged using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan)