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After incubation, cells were washed three times with HBSS, and the fluorescence signal was measured using a Spark Multimode Reader (Tecan, Mnnedorf, Switzerland)

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Worms were next transferred to room temperature NGM RNAi plates (100 g/mL carbenicillin, 1 mM IPTG) that were either unseeded (fasting conditions) or seeded with freshly generated RNAi bacteria ( ad libitum control) for 24 h

We employ rigorous analytical testing for every batch