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Total RNA was isolated from tea samples as described before for the samples used in transcriptome sequencing, and cDNA was synthesized using PrimeScript RT Reagent Kit (Takara, Dalian, China)

Trans-omic integration highlights AKT1-linked metabolic signatures in advanced lipedema Given the broad metabolic impact of the phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, whose aberrant activation can modulate multiple metabolic processes through direct and indirect mechanisms, we next investigated the metabolic alterations in lipedema using untargeted metabolomics [49, 50]