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Description

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Matching of Tm values and optimization of annealing temperature were not required to ensure efficient amplification (see figure: Tolerance of different primer Tm values)

Elevated HMGB1 levels have been detected in the blood of patients, where it interacts with receptors such as RAGE and TLR4, forming complexes with IL-1 or LPS to activate proinflammatory signaling pathways (Shimizu et al., 2017)