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Melittin, a small protein consisting of 26 amino acid residues, appears to downregulate phospholipase A2 (PLA2), cyclooxygenase (COX-2), and TNF- expression while reducing levels of IL-1, IL-6, nitric oxide (NO), and reactive oxygen species (ROS) (45)

Tested in WB, IHC, IP applications

Freezing a liquid solution causes sharp ice crystals to form

10.3389/fphar.2022.873646 237 YeS.MaoB.YangL.FuW.HouJ

Hence, it is logical to hypothesize that masking of the terminal NH 2 group by making a labile chemical derivative of INH, i.e., a derivative which can release INH back when exposed to biofluid in gut or blood (owing to hydrolytic activity of esterase/amidase) can limit known metabolic biotransformations of INH (Figure 6)