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Plasmolysing solution: 5 mM MES, 1 mM KH2PO4, 0.44 M D-sorbitol, 5 mM CaCl2, 2 mM MgCl2, 2 mM MnCl2, 1 mM L-arginine, 1 mM dithiothreitol (DTT), 0.1% (w/v) polyvinylpyrrolidone (PVP-10), 2 mM glutathione (GSH), 2 mM L-ascorbic acid, 0.01% (w/v) soybean trypsin inhibitor, 1300 U /ml catalase, adjusted to pH 5.7

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Some peptides, such as growth hormone-releasing peptides, stimulate the pituitary gland to produce additional growth hormone