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Western blot analysis Cells were washed with PBS containing 1 mmol L 1 N-Ethylmaleimide (NEM, Santa Cruz, #sc-202719), then lysed with Laemmli Sample buffer containing 2 % SDS, 4 % glycerol, 40 mmol L 1 Tris-Cl (pH 6.8), 5 % 2-Mercaptoethanol, 0.01 % bromophenol blue, 1 mmol L 1 NEM, 1 mmol L 1 Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor mixture (Roche, #COEDTAF-RO), and phosphatase inhibitor mixture (Roche, #04906837001)

Novel glutathione-dependent thiopurine prodrugs: evidence for enhanced cytotoxicity in tumor cells and for decreased bone marrow toxicity in mice

This is exactly why we include vitamin C in our detox drips

[DOI] [PubMed] [Google Scholar] Rudolf J

Detoxification: Supports liver function and aids in eliminating toxins