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Oxidative stress in neurodegeneration: cause or consequence

In general, affinity chromatography can be effectively used to isolate a protein that recognizes group X by:covalently attaching X or a derivative of it to a column, adding a mixture of proteins to this column, which is then washed with buffer to remove unbound proteins, eluting the desired protein by adding a high concentration of a soluble form of X or altering the conditions to decrease binding affinity

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doi:10.1016/S1097-2765(03)00053-4 39

However, the combined effects could theoretically impair beneficial swelling responses necessary for immune defense, though this has not been saw in research uses