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If the gene is expressing very early in life, its easy to spot

Quantitative PCR assays were performed using 480 SYBR Green Master reagent mix (Roche Life Science, 4707516001, murine probes) or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science)

A prospective in silico analysis of interdisciplinary and interobserver spatial variability in post-operative target delineation of high-risk oral cavity cancers: Does physician specialty matter