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Target protein purification yield was quantified by measuring the concentration of purified sample using the Bradford method (Coomassie Plus Bradford Assay Reagent, Pierce Biotechnology, Rockford, Ill.)

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- Film-type glutathione makes the active ingredients absorbed faster

These limitations are further amplified by the persistent male bias in metabolic and carcinogenesis studies of liver disease, which hinders identification of sex-specific mechanisms and limits the applicability of preclinical findings to humans

This helps stabilize results and prevent rebound weight gain