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Measurement of Enzymatic Activities The GSH-conjugation activity was assayed toward phenethyl-isothiocyanate (PITC), benzyl-isothiocyanate (BITC), 1-chloro-2,4-dinitrobenzene (CDNB), 4-hydroxy-2-nonenal (HNE), and 4-nitrophenyl-butyrate (PNP-butyrate) at 25C in a final volume of 500 L as described previously (Pgeot et al., 2014)