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Briefly, samples were liquefied at 37 C for 30 minutes, visualized by wet mount, vortexed and 50 uL was aliquoted for Computer Assisted Sperm Analysis

Together, these findings demonstrate that, in contrast with acute exposure, chronic disruption of iron homeostasis with consequent GSH depletion remodels cellular redox homeostasis over time, inducing a state we term chronoferroptosis: a persistent ferroptotic adaptation characterized by coordinated alterations in iron-handling and antioxidant defense proteins that may represent early vulnerability to neurodegenerative pathology

A 7.5 mg dose at this concentration means injecting 1.5 mL, which is at the upper limit of what most people find comfortable for a subcutaneous injection

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Frontiers in Psychiatry