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Recent studies have shown that, beyond eliminating pathogens through traditional phagocytosis, neutrophils participate in anti-infective defense and inflammatory responses by releasing neutrophil extracellular traps (NETs), secreting inflammatory mediators (e.g., cytokines, chemokines, and lipid mediators), and producing ROS (103, 104)

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51 Monoamine oxidase (MAO) activates the metabolism of adrenaline and triggers calcium signaling in astrocytes, 52 which suggests that antidepressants may directly affect astrocytes by preventing them from reabsorbing monoamines

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The Gateway destination vector pAG415GAL-ccdB (CEN/ARS-based, GAL1 promoter, and LEU2 marker) was purchased from Addgene and served as the yeast expression vector

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