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In brief, serum-free keratinocyte growth medium (1, Gibco) was supplemented l-Glutamine, 5 ng ml 1 epidermal growth factor and 0.05 mg ml 1 bovine pituitary extract (BPE), 500 ng ml 1 hydrocortisone and 0.005 mg ml 1 insulin (Gibco)

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Rather than allowing peptide use to remain confined to nonmedical distribution channels, orthopedics should lead the development of standardized perioperative registries and prospective trials using verified formulations, with the goal of determining whether peptide-based therapies can be safely integrated into evidence-based surgical optimization

Dangers and uncertainties still loom