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Because the FAT10 UBL1 domain enters the proteasome first, the position of the FAT10 conjugation site determines the starting point for unfolding and translocation on a substrate, requiring the proteasome to potentially process a branch point and thread two strands through the central channel

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o , Parental HEK293T cells or pools of cells treated with gRNAs/Cas9 complexes targeting MYH9 and MYH10 were transfected with TMEFF1 and infected with HSV-1(MOI 0.5)