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Cells were treated with PEMS buffer containing 1% Triton X-100 at room temperature for 1 min, washed twice with PEM buffer, and incubated in PEMBAL buffer (100 mM PIPES pH 6.9, 1 mM MgCl 2 , 1 mM EGTA, 1 % BSA, and 0.1 M L-lysine) at room temperature for 1 h with gentle rotation

Notably, a single APX transcript (HORVU.MOREX.r2.2HG0088330.1) displayed very high and stable expression across all ME stages and in pollen, reaching 1000 FPKM ( Figures 3A, B , Supplementary Figure S3B )

gametophytic control) showed SOD levels comparable to induction stages

doi: 10.3390/ijms22179459 107 ZulloAHassanCCristofariFAndrianiADe FrancescoVIerardiEet al