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Host mice were immunized subcutaneously in the hock area (50 l each site) in both legs with 1 mg/ml -1 ovalbumin (OVA) 323339 peptide (InvivoGen) emulsified with complete Freund adjuvant (CFA

Generally, iron accumulation induces OS, bringing about the increase of ROS levels and the imbalance of antioxidant balance and infiltrating pro-inflammatory mediators that further amplify cerebral inflammatory responses, which aggravate neuronal damage during reperfusion (Jin et al., 2013

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Eyes were then washed, dehydrated and embedded in paraffin wax

Cell Physiol Biochem 37(3):10291043 Zhang L, Liu H, Zheng Y (2024) Exploration of major depressive disorder among children and adolescents: from pathogenesis to intervention