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2.11 Cck-8 assay Cells (1000 per well) were incubated into 96 well and cell vitality was assessed by Cell Counting Kit-8 (Dojindo, Cat.no

Asian-australas J Anim Sci (2009) 22(10):146176.10.5713/ajas.2009.80659 28 HorioMItoAMatsuokaYMoriyamaTOritaYTakenakaMet alApoptosis induced by hypertonicity in Madin Darley canine kidney cells: protective effect of betaine