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Am J Med Sci 1949

To investigate whether the increased GSH level at 100 g/ml 5-FOA (Figure S2A) was stable during longer-term cultivation of the modified strain, cellular GSH content was monitored every 10 generations during sequential batch cultivation in flasks at 100 g/ml 5-FOA (after every ~ 10 generations, aliquots of overnight culture were transferred to fresh medium giving OD 0.01 and re-incubated)

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If you dont have a stirrer, repeatedly pour the paint and water from one 5-gallon bucket to another 5-gallon bucket until its evenly mixed