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Vitamin C and glutathione work together in a recycling loop

[73] PPIs are prodrugs and weak bases that are activated in very acidic environment

Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)