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X-ray crystallography Complex formation was performed by mixing TEV-cleaved DAM21 or DAM27 (1.5 eq.) with refolded, synthetic d-Abl SH2 A198C (1 eq.) and subsequent purification of each complex by SEC on a HiLoad 16/600 Superdex 75 pg column (Cytiva) column using the kta Avant system (Cytiva) into 20 mM HEPES, 150 mM NaCl, 0.5 mM TCEP-HCl

To better understand the mechanism by which DDX3X modulates HSV-1 nuclear capsid egress, we next evaluated if C-capsids were needed for DDX3X nuclear localisation

Eder, M., Fritz-Wolf, K., Kabsch, W., Wallimann, T