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cDNA was made from isolated RNA using Bio-Rad iScript cDNA synthesis kits (Bio-Rad, Hercules, CA)

Winther KH, Wichman JEM, Bonnema SJ, et al

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For experiments involving more than two groups, statistical significance was evaluated using one-way analysis of variance (ANOVA), followed by Dunnett's multiple comparisons test, in which each treatment group was compared exclusively with the corresponding control group