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Maintenance of cells and animals Ea.hy926 (E9) cells: E9 cells were purchased from National Centre for Cell Science (NCCS), Pune and maintained in Dulbeccos Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Himedia, A002) (10,000 units of penicillin and 10 mg of streptomycin in 0.9% NaCl) in a humidified atmosphere with 5% CO 2 at 37 C

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The samples were loaded on the 96-well S-Trap plate (Protifi), placed on top of a deepwell plate, and centrifuged for 2 min at 1,500 x g at RT

This approach moves beyond non-specific chemotherapy combinations towards mechanism-informed drug partnerships [15]

Morphological changes, occurring during the activation of microglia and astrocytes, or higher proliferation of NG2 glia, microglia and astrocytes are all hallmarks of ischemia (Davalos et al., 2005