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Purification and Scale-Up Considerations After synthesis with various precursors, the yield contains impure CQDs with the presence of by-products, unreacted precursors, and impurities which might interfere with the characterisation, potentially leading to experimental error [79]

To circumvent this difficulty and to reduce the possibility of suppressor mutations that compensate for chronic loss of V-ATPase structural or assembly genes, we fused an AID* degron domain (a 71-114 truncated fragment of the IAA17 protein that proved to work efficiently in budding yeast) 24 to the endogenous Vma2 protein

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