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Vimentin is a non-essential gene in many cell lines, and we use it as a positive control that it can be knocked out in TaC12 and BoMac cells

These heal leaving areas of hyperpigmentationand hypopigmentation

Cao H, Duan L, Zhang Y, et al

(2006) used concentrations of 1500 mg/kg of MTX in female rats after birth and found that ovarian weight decreased with treatment at 50, 100, and 500 mg/kg