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The results of the study showed that treatment with AGN extract improved the mean weight of the cryptorchid testis, prevented oxidative stress in TM3 cells in a dose-dependent manner, preserved sperm counts, motility, and spermatogenic cell density, reduced 8-hydroxy-2-deoxyguanosine (8-OHdG) and increased superoxide dismutase (SOD) levels, considerably raised Nrf2 and HO-1, as well as substantially lowered apoptosis

In addition, multiple transcription factors, such as ATF3, ATF4, P53, Nrf2, and c-Myc, have been identified as the regulators of SLC7A11 and SLC3A2 transcription [11, 27,28,29,30]

Available inducers (e.g., erastin, RSL3, elesclomol) often suffer from poor pharmacokinetics, limited tumor selectivity, and narrow therapeutic windows, leading to potential off-target toxicity