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the pellet, corresponding to blood cells, was washed two times with 0.9% NaCl and was frozen at 80 C until analysis

This is particularly relevant when we consider GSH pathways, as well as their transcriptional regulator Nrf2, for proliferation, survival and function of T cells, B cells and macrophages (Muri and Kopf, 2021)

Additionally, polymorphisms in diamine oxidase, an enzyme critical in histamine breakdown, may affect the outcome of immune-modulator therapeutics in IBD patients