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In one study of 127 young mean age, 20.3 years) women, supplementation with a high dose of thiamin (50 mg/day

RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

CNS & Neurological Disorders-drug Targets 16:936944 Colwell CS, Levine MS (1995) Excitatory Synaptic Transmission in Neostriatal Neurons: Regulation by Cyclic AMP-Dependent Mechanisms

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The dosage schedule of DPA is: adults 250 mg qid, po, for 1-2 weeks, children 20-30 mg/kg/daily in 4 divided doses (maximum 250 mg/dose)