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One disadvantage of this method is that the hemocytometer instrument used for cell counting is prone to error, and the other is that it has a negative effect on mammalian cells [19]

Int J Sport Nutr Exerc Metab 2016

Mn preferentially accumulates in mitochondria, through the mitochondrial Ca 2+ uniporter, where it is mainly bound to mitochondrial membrane or matrix proteins ( 2+ binds to these substrates effectively inhibiting mitochondrial respiration (Wang et al., 2015)