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doi:10.1016/j.meegid.2008.01.002

Methods Cloning and purification Three hTrxR1 constructs were cloned into the pET28a(+) expression vector (Novagen) using Nde I and Hin dIII restriction sites: a double hTrxR1 mutant where Cys497 and Sec498 were replaced by Ser and Cys, respectively (hTrxR1 C497S,U498C ), as well as a hTrxR1 mutant where Sec498 was replaced by Ser (hTrxR1 U498S ), and a truncated form of hTrxR1 where a stop codon was introduced after Cys497 (hTrxR1 C497_trunc )

elegans neurons, promoter sequences of cat-2prom3 (1143 bp), dat-1 (786 bp), hlh-17 (3008 bp), or elt-2 (5049 bp) were inserted into the pSM::SL2::GFP:: unc-54 3 UTR vector along with the cat-2 genomic coding region

Education and training on every compound

Eligible DCD patients had longer withdrawal to asystole times than non-eligible patients (p 0.05)