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Proteins were eluted by incubation with 25 L of 100 g/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp) in IP buffer on an end-over-end tube rotator at 10 rpm at 4 C overnight

Why It Was in Category 2 Despite decades of clinical use in Russia (where it is an approved pharmaceutical), Semax was placed in Category 2 in the US due to safety concerns, likely related to the limited data from US-standard clinical trials and the regulatory gap between international evidence and FDA requirements

Cruz, M

In Mouse Liver Neoplasia (Popp J