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When dietary restriction mice were fed EAAs but not non-essential amino acids, the effect of dietary restriction-dependent longevity prolongation was reduced (Yoshida et al., 2018)

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10.3390/ijms21010243 [DOI] [PMC free article] [PubMed] [Google Scholar] 42.Takenaka K, Fujii T, Kawamoto A, Suzuki K, Shimizu H, Maeyashiki C, Yamaji O, Motobayashi M, Igarashi A, Hanazawa R, Hibiya S

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Melting curve analysis was performed (0.5 C/s increase from 55 to 95 C with continuous fluorescence readings) at the end of 40 cycles to ensure that specific PCR products were obtained