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Cells were plated in 96-well plates at an appropriate density depending on the cell line (between 1000 and 10 000 cells/well in 10OpL of appropriate culture media) and incubated at 37C for 24 hours

Finally, we outline future directions within an ethnopharmacological perspective, integrating traditional knowledge with pharmacological innovation to inform multi target, standardized, and potentially personalized interventions for defined ASD endophenotypes

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Overactivation of stress/trauma-related circuits results in glutamate spillover and excitotoxicity (Averill et al

Food Sci Biotechnol 28:529537