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Measurements from the 1966 study had shown elevated chloride concentrations in the Flint River, still true in 2014

AGC target value for fragment spectra was set at 1E5 with a resolution of 15,000 and injection times of 22 ms and Top12

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Flow cytometry was performed on the DPCs, and quantification was done using Image J Pro software

Besides, to further confirm peroxynitrite-mediated activation of the NF-B pathway, we also measured the mRNA expression levels of known NF-B p65 target genes Bax , P53 , Bcl2 , and Bcl-xL