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Subsequently, Western Blot (WB) analysis detected a decrease in total P53 expression but an increase in phosphorylated P53 (p-P53) expression in OGD-induced senescent endothelial cells post FOXO4-DRI treatment (Figures 5B,C)

Cysteine availability is often the rate limiter for glutathione synthesis, and thus supplementing with NAC increases net glutathione levels, which is useful in disease states characterized by ischemia, increased oxidative stress, and reduced available glutathione [210, 211]

Gwathmey, Zhenghang Zhao Pflgers Archiv - European Journal of Physiology.2021

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Mechanisms and biochemical bases The following section examines the biochemical basis of the glucose-alanine cycle, with particular emphasis on the metabolic interplay between skeletal muscle and the liver