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The resulting supernatant was then separated and stored at 80C for further colorimetric measurements with a microplate reader (BioTek Synergy H1, Agilent, USA)

Vrdoljak J, Kumric M, Vilovic M, Martinovic D, Tomic IJ, Krnic M

APAP-induced liver injury is associated with glutathione depletion, the formation of APAP protein adducts, the generation of reactive oxygen and nitrogen species and mitochondrial injury