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Description

We dissect each clathrin box by mutagenesis to track their occupancy by the Ent1 and Ent2 adaptors and show that both Ent1 and Ent2 exhibit different Chc binding patterns, as revealed by native MS and photobleaching FRET assays

To identify such factors, we screened the previously obtained list of putative FBP interactors from U-2 OS lysate for proteins linked to Rac1 activation