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After removing the supernatant, the resulting pellet was resuspended in 200 l of B-PER reagent (supplemented with 1 mM DTT and 1 mM PMSF)

This phase 2 study compared brodalumab against placebo in patients with active PsA (both non-exposed and exposed to prior biologic therapy) to a primary endpoint of 20% improvement of American College of Rheumatology (ACR) criteria at week 12

Unlike many other vitamins, B12 is stored in the liver in substantial quantities, meaning deficiency typically develops gradually over months to years as these reserves become depleted

Abbreviations AchE: Acetylcholinesterase AdoMet: S-adenosylmethionine AD: Alzheimers disease AFM: Atomic force microscopy A: Amyloid-beta BchE: Butyrylcholinesterase Bcl-2: B-cell lymphoma BHK: Baby hamster kidney BSA: Bovine serum albumin CAM: Chicken chorioallantoic membrane DCFH-DA: 2,7-Dichlorodihydrofluorescein diacetate DTPA: Diethylenetriaminepentaacetate CD: Circular dichroism CHIKV: Chikungunya virus ChEIs: Cholinesterase inhibitors CSF: Cerebrospinal fluid dsRNA: Double-stranded RNA EB: Ethidium bromide ECM: Extracellular matrix FAAS: Flame atomic absorption spectroscopy -Gly: -Glycosidase H 2 O 2 : Hydrogen peroxide HO : Hydroxyl HIF-: Hypoxia-inducible factor IZD: Inhibition zone diameter MBC: Minimum bactericidal concentration MIC: Minimum inhibitory concentration MMP: Mitochondrial membrane potential MFC: Minimum fungicidal concentration MSR: Methionine synthase reductase MoM: Metal-on-metal nsP4: Non-structural protein 4 O 2 : Superoxide RBD: Receptor binding domain SEM: Scanning electron microscopy SI: Selectivity index ROS: Reactive oxygen species TEM: Transmission electron microscopy UTP: Uridine 5-triphosphate VSV: Vesicular stomatitis virus VZV: Varicella-zoster virus References Chang EL, Simmers C, Knight DA (2010) Cobalt complexes as antiviral and antibacterial agents

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