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Acknowledgements The authors gratefully acknowledge the support and encouragement provided by the School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India

Apr 2021;115(4):1063-1073

Pediatrics 145, S225-S230S230

Newly synthesized Nrf2 then accumulates and translocates to the nucleus, where it binds to antioxidant response elements (AREs) to drive the transcription of cytoprotective genes, including SLC7A11 (which drives cystine import) as well as glutamate-cysteine ligase and glutathione synthetase, which collectively orchestrate the de novo synthesis of GSH [68-70]

One-step reverse transcription was performed using the Reverse Transcription Kit (#AT301, TransGen, Beijing, China) according to the manufacturers protocol